Product ID:HUCKER AMMONIUM OXALATE CRYSTAL VIOLATE(SEE SUPPLEMENTAL)
MSDS Date:09/05/2000
FSC:NIIN:Submitter:D DG
Status Code:A
MSDS Number: CLDCG

CAROLINA BIOLOGICAL SUPPLY CO

2700 YORK RD
BURLINGTON, NC 27215-3387
US

Emergency Phone: 800-227-1150

Info Phone: 800-227-1150

Cage: 59896

CAROLINA BIOLOGICAL SUPPLY CO

BURLINGTON, NC 27215-3387 US

Telephone: 800-227-1150/910-584-0381

Cage: 59896

CRYSTAL VIOLET STAIN

CAS: 548-62-9

RTECS: BO9000000

AMMONIUM OXALATE

CAS: 1113-38-8

RTECS: RO2750000

ETHYL ALCOHOL (ETHANOL)

CAS: 64-17-5

RTECS: KQ6300000

OSHA PEL 1000 PPM

ACGIH TLV: 1000 PPM; 9596

METHYL ISOBUTYL KETONE (HEXONE) (SARA 313) (CERCLA)

CAS: 108-10-1

RTECS: SA9275000

OSHA PEL 100 PPM

ACGIH TLV: 50 PPM/75 STEL; 9596

EPA Report Quantity: 5000 LBS

DOT Report Quantity: 5000 LBS

HEXANE (N-HEXANE) (CERCLA)

CAS: 110-54-3

RTECS: MN9275000

OSHA PEL 500 PPM

ACGIH TLV: 50 PPM; 9596

EPA Report Quantity: 1 LB

DOT Report Quantity: 1 LB

Routes of Entry: Inhalation:YES Skin:YES Ingestion:YES
Reports of Carcinogenicity:NTP:NO IARC:NO OSHA:NO
Health Hazards Acute and Chronic:TARGET ORGANS: SKIN, EYES, NOSE,
THROAT. EYES: MAY CAUSE IRRITATION. SKIN: MAY CAUSE IRRITATION.
INHALATION: MAY CAUSE IRRITATION TO RESPIRATORY TRACT. INGESTION:
MAY CAUSE GASTROINTESTINAL DISCOM FORT.
Effects of Overexposure:EYES: IRRITATION. SKIN: IRRITATION. INHALATION:
IRRITATION TO RESPIRATORY TRACT. INGESTION: MAY CAUSE
GASTROINTESTINAL DISCOMFORT.
Medical Cond Aggravated by Exposure:PRE-EXISTING CONDITIONS OF THE
SKIN, EYES, THROAT.

First Aid:EYES: FLUSH WITH WATER FOR AT LEAST 15 MIN, RAISING &
LOWERING EYELIDS. SKIN: THOROUGHLY WASH FOR 15 MIN. REMOVE
CONTAMINATED CLOTHING. INGESTION: IF CONSCIOUS, GIVE PLENTY OF
WATER & INDUCE VOMITIN G IMMEDIATELY AS DIRECTED BY DOCTOR.
IMMEDIATELY CALL A PHYSICIAN OR POISON CONTROL CENTER. NEVER GIVE
ANYTHING BY MOUTH TO AN UNCONSCIOUS PERSON. INHALATION: REMOVE TO
FRESH AIR. GIVE OXYGE OR ARTIF ICIAL RESPIRATION AS REQUIRED. KEEP
PERSON WARM & QUIET. GET MEDICAL ATT'N AS NEEDED FOR ALL ROUTES OF
EXPOSURE.

Flash Point Method:CC
Flash Point:=13.1C, 55.6F
ETHYL ALCOHOL
Lower Limits:3.3,ETHYL AL
Upper Limits:19, ETHYL AL
Extinguishing Media:USE DRY CHEMICAL, CARBON DIOXIDE, OR APPROPRIATE
FOAM.
Fire Fighting Procedures:FIREFIGHTERS SHOULD WEAR FULL PROTECTIVE
EQUIPMENT AND NIOSH APPROVED SELF-CONTAINED BREATHING APPARATUS.
AUTOIGNITION TEMPERATURE FOR ETHYL ALCOHOL IS 793F.
Unusual Fire/Explosion Hazard:IF HEATED TO DECOMPOSITION, THIS MATERIAL
MAY EMIT OXIDES OF CARBON. DUE TO THE DILUTION OF THE FLAMMABLE
MATERIAL IN THIS MIXTURE IS NOT LIKELY TO POSE A SERIOUS FIRE OR
EXPLOSION THREAT. HOWEVER, IF EXPOSED TO EXCESSIVE HEAT, FLAMMABLE
VAPORS MAY BE RELEASED.

Spill Release Procedures:VENTILATE AREA OF SPILL. ELIMINATE ALL SOURCES
OF IGNITION. REMOVE ALL NON-ESSENTIAL PERSONNEL FROM AREA. CLEAN-UP
PERSONNEL SHOULD WEAR PROPER PROTECTIVE EQUIPMENT AND CLOTHING.
ABSORB MATERIAL WITH SUITABLE ABSORBENT AND CONTAINERIZE FOR
DISPOSAL.

Handling and Storage Precautions:STORE TIGHTLY CLOSED IN A COOL, DRY,
WELL VENTILATED AREA SUITABLE FOR GENERAL CHEMICAL STORAGE.
Other Precautions:NONE

Respiratory Protection:A NIOSH/MSHA CHEMICAL CARTRIDGE RESPIRATOR
SHOULD BE WORN IF PEL OR TLV IS EXCEEDED.
Ventilation:LOCAL EXHAUST PREFERRED, MECHANICAL (GENERAL) ACCEPTABLE.
Protective Gloves:RUBBER, NEOPRENE, PVC, OR EQUIVALENT.
Eye Protection:SPLASH PROOF CHEMICAL SAFETY GOGGLES SHOULD BE WORN AT
ALL TIMES.
Other Protective Equipment:EYE WASH, LAB COAT, AND SAFETY SHOWER.
Supplemental Safety and Health
PART NUMBERS: 82-1050, 86-7483, 89-2301, 15-4708, 15-4708A, 15-4718,
15-4724A, 15-4708CA.

HCC:V5
Vapor Pres:NO INFORMATION AVAILABLE
Spec Gravity:<1
Evaporation Rate & Reference:NO INFORMATION AVAILABLE.
Solubility in Water:COMPLETE
Appearance and Odor:DEEP PURPLE SOLUTION WITH ODOR OF ETHANOL.
Percent Volatiles by Volume:97 APPROX

Stability Indicator/Materials to Avoid:YES
WATER REACTIVE MATERIALS, STRONG OXIDIZERS.
Stability Condition to Avoid:EXCESSIVE HEAT, FLAME.
Hazardous Decomposition Products:IF HEATED TO DECOMPOSITION, MAY EMIT
OXIDES OF CARBON AND NITROGEN.
Conditions to Avoid Polymerization:WILL NOT OCCUR.

Waste Disposal Methods:DISPOSE IN ACCORDANCE WITH ALL APPLICABLE
FEDERAL, STATE, AND LOCAL REGULATIONS. ALWAYS CONTACT A PERMITTED
WASTE DISPOSER (TSD) TO ASSURE COMPLIANCE.

Toxicological Information:TOXICOLOGICAL PROPERTIES OF THIS MIXTURE HAVE
NOT BEEN THROUGHLY EVALUATED. DATA IS LISTED FOR INDIVIDUAL ITEMS.
CRYSTAL VIOLET STAIN - (ORAL-RAT) = 420MG/KG. AMMONIUM OXALATE- NO
TOXICITY DATA. ETHYL ALCOHOL - LD50 (ORAL-RAT) - 7,060 MG/KG; LC50
(IHL-RAT) = 8,000 PPM/4H. HEXANE: LD50 (ORAL-RAT) = 28,710 MG/KG,
(IHL-HUMAN) - 5,000 PPM/10M. CRYSTAL VIOLET STAIN: MUTATION
REFERENCES CITED. REPRODUC TIVE EFFECTS REFERENCES CITED.
TUMORIGENIC REFERENCES CITED. AMMONIUM OXALATE: NO CHRONIC EFFECTS
DATA FOUND. ETHYL ALCOHOL: MUTATION DATA CITED. METHYL ISOBUTYL
KETONE: NO CHRONIC EFFECTS CITED. HEXA NE: MUTATION DATA CITED.

Ecological:EPA WASTE NUMBERS: METHYL ISOBUTYL KETONE (U161)

Transport Information:NON-REGULATED MATERIAL.

SARA Title III Information:HAZARD CATEGORY FOR SECTION 311/312
REPORTING: ACUTE. SARA SEC. 313: CRYSTAL VIOLET STN, AMMONIUM
OXALATE, ETHYL ALCOHOL, AND HEXANE: SARA EHS SEC. 302 TPQ: NO;
SARA SEC 313 CHEMICALS NAME LIST= NO; CHEMICAL CATEGORY=NO; CERCLA
SECTION 103 RQ LBS= NO; RCRA SEC 261.33=NO. METHYL ISOBUTYL KETONE:
SARA EHS SEC 302 TPQ=NO; SARA SEC 313 CHEMICALS NAME LIST= YES;
CHEMICAL CATEGORY = NO ; CERCLA SEC 10 3 RQ LBS= 5000 LBS.; RCRA
SEC 261.33 = YES.
Federal Regulatory Information:EA TSCA STATUS: ON TSCA INVENTORY.

Disclaimer (provided with this information by the compiling agencies):
This information is formulated for use by elements of the Department
of Defense. The United States of America in no manner whatsoever,
expressly or implied, warrants this information to be accurate and
disclaims all liability for its use. Any person utilizing this
document should seek competent professional advice to verify and
assume responsibility for the suitability of this information to their
particular situation.